Question 1

Why is resolution important in chromatography?

Accepted Answer

Resolution is critical because it determines whether individual compounds can be accurately quantified without peak overlap.

Question 2

What is the difference between tR and t0?

Accepted Answer

tR (retention time) is the time elapsed between injection and the peak maximum of the analyte, while t0 (void time) is the time taken for an unretained solvent front to pass through the column.

Question 3

Can I use this calculator for both GC and HPLC?

Accepted Answer

Yes, the fundamental principles of column efficiency and retention apply universally across both gas and liquid chromatography techniques.

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